Highly Interactive Course

Oilfield Microbiology

Manage Asset Integrity, Safety, and Environmental Problems

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Venue Starting Date Duration Fees Join Now
Online - Virtual , 20240722 22 - Jul - 2024 2 Hrs X 8 Sessions $2,100 Register
Dubai - UAE , 20240826 26 - Aug - 2024 5 - Day $4,950 Register
Kuala Lumpur - Malaysia , 20241007 07 - Oct - 2024 5 - Day $5,950 Register
Online - Virtual , 20241118 18 - Nov - 2024 2 Hrs X 8 Sessions $2,100 Register
Dubai - UAE , 20241216 16 - Dec - 2024 5 - Day $4,950 Register


Oilfield microbiology is an often-overlooked aspect of production chemistry. Uncontrolled microbial activity in oilfields can lead to significant asset integrity, safety, and environmental problems, which cost a lot. Their leading causes are reservoir souring, microbiologically influenced corrosion (MIC), and process upsets. These issues can be avoided by taking a proactive approach to microbial monitoring and mitigation to save money and improve production.

This Oilfield microbiology course covers the basic problems that microorganisms cause, including reservoir souring, MIC, and process control upset. You will know how best to monitor and mitigate microorganisms from both a theoretical and practical point of view through case study classroom work and laboratory microbial monitoring and testing techniques. Participants will have a complete understanding of the causes of microbial reservoir hydrogen sulphide souring and the actions and processes needed to reliably predict, monitor, control, and manage the risks of souring.


Upon completion of this Oilfield microbiology training course, participants will:

  • Understand the different types of microorganisms.
  • Learn microbiological processes as applied to the reservoir.
  • Determine how they cause souring and corrosion.
  • Develop an understanding of the various microbial souring prediction tools available.
  • Assess data supplied on existing biocide formulations and other microbial control measures appropriate to the system.
  • Identify solutions for monitoring and mitigation programmes.
  • Be able to establish a proper souring monitoring program for the operation.
  • Be able to interpret field data on microbiology and souring accurately,
  • Recognizing how to assess the effectiveness of any applied souring mitigation strategy
  • Determine and recommend any needed changes to the strategy, including biocide evaluation and optimization.
  • Set up appropriate sampling locations.
  • Take accurate samples and learn preservation techniques for further laboratory analysis.


This course is suitable for a wide range of professionals but will greatly benefit:

  • Reservoir Engineers, Production Engineers, Operations Personnel, Technical Personnel, Management Personnel and Research & Development Personnel
  • Anyone who is controlling and managing microbial reservoir souring.
  • Anyone wishing to improve their understanding of microbes in the oil industry


This Oilfield microbiology course utilises various proven adult learning techniques to ensure maximum understanding, comprehension, and retention of the information presented. The programme will be interactive and practical. There will be work in groups and pairs as well as individual exercises, and everyone will get an opportunity to discuss his issues.

Pre & Post course assessments will be used to measure the effectiveness of this training.


Module 1 - Oilfield Microbiology Overview and Corrosion

  • KeyTopics:

    • Introduction to Oilfield Microbiology
      • Overview of microorganisms
      • The oil field and petroleum microbiology
      • Bacteria growth and survival
      • Biofilms and microbial activity
      • Microbes encountered in oilfield systems
      • Overview of problems caused by microorganisms
      • Positive impacts of microbes in the oil industry
    • Microbiologically Influenced Corrosion (MIC)
      • Introduction to MIC
      • The difference between MIC and other corrosion
      • Classification (types of corrosion)
      • Other causes of corrosion
      • Microorganisms related to MIC ex (SRB, GAB,….etc)
      • Microbiologically influenced corrosion (MIC)
      • Assessment and mitigation of MIC

Module 2 - Microbial Sampling and Control Methods

  • KeyTopics:

    • Microbial Sampling, Identification & Monitoring
      • Classical and new approaches to microbiological monitoring
      • Sampling procedures for microbiology covering water, solids, and surfaces
      • Good sampling practices
      • How to sample water, oil, and solids
      • Importance of on-site sampling and representative samples
      • Samples preservation
      • Scales types & analysis
      • Microbial analysis techniques
      • Implementing a microbial monitoring plan
      • Cost-effective Plan.
    • Process control issues caused by microorganisms:
      • Where microorganisms are commonly found in:
        • Injection, production, and water treatment systems
      • The problems you might not expect.

Module 3 - Microbial Sampling and Mitigation Measures

  • KeyTopics:

    • Microbial Sampling, Identification & Monitoring
      • The common error in monitoring
      • Mitigation options
      • Importance of cleaning and intelligent pigging
      • Chemical disinfection
      • Resistance to chemical action
      • Biocide testing and evaluation
      • Control in sea water injection systems
      • Control in non-sea water injection systems
      • H2S scavenging, Methods and protocols
      • Molecular microbiology methods
      • Traditional microbial cultivation methods
      • Quick kits for microbial assessment
      • Practical Detection of bacterial organisms in lab
    • Mitigation measures for different scenarios:
      • What to use where and more importantly
      • What not to use where.

Module 4 - Reservoir Souring Assessment and Prediction

  • KeyTopics:

    • Reservoir Souring: Why is it Important and How Does it Happen?
      • Souring of oil and gas fields – why, how, and when
      • Importance of souring – safety, sour service materials, product quality
      • Causes and consequences of microbial reservoir souring,
      • Chemical and physical requirements (nutrients, temperature, pressure)
      • Chemical calculations and chemical pump adjustments
      • Which organisms are responsible?
      • The likelihood of souring
      • Assessment of biogenic reservoir souring
      • Are they indigenous or introduced?
      • Seawater injection – the usual cause
      • Reservoir souring and predictions
      • Predictive modeling
      • Field planning to mitigate reservoir souring
      • Effect of reservoir structure and geology on souring
      • Scavenging of H2S by Iron minerals in reservoirs
      • Partitioning of H2S between oil, water, and gas
      • Effect of GOR & water cut on perceived souring
      • Reservoir souring case studies
    • Reservoir Souring Assessment and Prediction
      • Using downhole samples
      • Importance of a fixed planned sampling campaign
      • Calculating mass balance of H2S – importance and methods
      • H2S generation rates estimated from laboratory experiments
      • Measurement of H2S in the field, all 3 phases
      • How to predict the potential for souring before field development
      • The biofilm souring model
      • History matching of souring

Module 5 - Souring Controls & Microbial-enhanced Oil Recovery (MEOR)

  • KeyTopics:

    • Controls of Souring and Case Histories
      • Biocides used in water injection systems
      • Biocides designed for downhole use
      • Why standard biocide control treatments may not work
      • Novel microbial control methods
      • Nitrate – sometimes good, sometimes not
      • H2S scavengers
      • Industry standards for souring
    • Microbial-enhanced Oil Recovery (MEOR)
      • Introduction
      • MEOR applications
      • Microbes used in MEOR
      • Screening criteria
      • Putative MEOR mechanisms
      • Souring risk from MEOR treatments
      • Worldwide cases of souring
      • Biocide reservoir treatments
      • Nitrate reservoir treatments
      • Impact of other production chemicals
      • Microbial control methods – biocides
      • Microbial control methods – novel and developing methods

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